AWS Activate credit: Eligibility criteria

No , I don't think it's a must meet condition. The Eligibility Criteria Per given link are https://aws.amazon.com/startups/credits#packages

Associated with an Activate Provider and have their Organizational ID

Have not previously redeemed $100,000 total in AWS Activate credits

Have not previously received AWS Activate credits of equal or greater value from an Activate Provider

Self-funded or funded pre-series B

Have a fully-functioning company website

Founded in the past 10 years

Would strongly recommend to contact the respective team to get formal and detailed answer (https://aws.amazon.com/activate/contact-us/) . Answers on public platform like repost are at times best to the knowledge and might not cover edge cases per individual situation.