Create MD5 Checksum with Mediaconvert

The MediaConvert service does not internally support this however the larger AWS environment can easily do this.

Any S3 bucket can be configured to emit S3 File events when a new file arrives --including when MediaConvert delivers new output files. These S3 File Events can trigger various actions such as a Lambda Function, which can compute the Md5 hash of the target file and record that has value somewhere, such as a key/value pair Tag on the new file, or a database table etc.

This tutorial covers the S3 File Events-->Lamba workflow: https://docs.aws.amazon.com/lambda/latest/dg/with-s3-example.html

Alternatively you could run the md5 compute/tag process in a batch operation or once-daily job, depending on requirements or preference.