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Check your route table has the correct IP route in your vpc/subnet route table back to the lightsail VPC. You will need to add one as this isn’t created automatically.
Without a route, the return traffic will follow the default route or one that has a better preference.
1
Is the Lightsail IP address set in the security group a private IP address?
Access via VPC peering, so if Lightsail's private IP address is not configured, you will not be able to connect.
Also, check again that you have a peer connection to the correct VPC where RDS is running.
It is also recommended to check the network ACLs used on the RDS subnet to be sure.
FYI By default light sail uses 172.26.0.0/16 as the VPC cidr.
Details here https://lightsail.aws.amazon.com/ls/docs/en_us/articles/lightsail-how-to-set-up-vpc-peering-with-aws-resources
Not sure why AWS SP marked the answer down when this is the right answer.