Hi, did you check the public key configured in the EC2 instance to validate that it corresponds to your .pem file for the private key ? I would start there to ensure that the EC2 is what I believe it is.
After that, you can try a console access to EC2 instance: a Windows terminal will be opened if successful. If it succeeds, this will validate your key and then it will boil down to an RDP config issue.
If all else fails you can take a snapshot and relaunch from it, providing new credentials you know are definitely OK.
It would be good to move to a more secure access mthod though - Fleet Manager RDP. The instance needs to have the SSM Agent running (which modern AMIs should have), and needs appropriate IAM role permissions. See https://docs.aws.amazon.com/systems-manager/latest/userguide/fleet-setup-iam.html.
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