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Hello,
The Security groups are just one of the factors needed to enable access. There are multiple other parameters like NACL's, Public IP, Routing etc that need to be verified between the 2 environments before reaching to a conclusion. Have you checked the routing that is set ?
Assuming, you are trying to access the same from On-Premise over the internet, did you verify if Internet gateway is attached to the routing in the EC2 instance. Please verify the same or provide more information on how is the routing set up.
Link- https://docs.aws.amazon.com/AWSEC2/latest/UserGuide/TroubleshootingInstancesConnecting.html
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