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Hello,
Check the actual system logs of the machine
Click on Actions > Monitor and troubleshoot > Get System Log. This will display the instance's boot log, which can provide insights if the instance failed to boot.
Have you tried stopping the instance and booting it up again ?
Also, check you are not blocking unintentionally ports on security groups and/or NACL.
Thanks
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I dont believe Security groups have any impact on EC2 instance checks as they are carried out on the underlying infrastructure and not via directly via the network interface