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Some quick checks you can verify
- Check for any missing values for your User Defined Variables like AWS access key, secret key, host, service name and region as applicable
- Check for proper header names with values
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asked 3 years ago
asked 3 years ago
- AWS OFFICIALUpdated 10 months ago

Are you certain jmeter isn't adding additional HTTP Headers that aren't included when generating the signature? I believe all or most of the HTTP Headers need to be factored into the signature.