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AppStream 2.0 is a managed service with managed gateways. The fleet, while sitting in a private subnet or more, are streamed through public Gateways. There is another ENI on fleet instances that are dedicated for streaming and service health, which you cannot attach Security Groups to. Now, there is the option to stream through a VPC Endpoint, forcing streaming traffic through a VPC interface - https://docs.aws.amazon.com/appstream2/latest/developerguide/creating-streaming-from-interface-vpc-endpoints.html
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