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Hello
Assuming there were no changes done to the ACL or security group rules that block TCP traffic but allow ICMP (Ping) more likely scenario is configuration within the instance is causing an issue. I would try getting into the instance to further debug the issue and since SSH via Putty is not an option you can try either accessing the instance via EC2 Connect or Session Manager.
- EC2 Connect Configuration -> https://docs.aws.amazon.com/AWSEC2/latest/UserGuide/ec2-instance-connect-set-up.html
- Session Manager -> https://docs.aws.amazon.com/systems-manager/latest/userguide/systems-manager-setting-up-ec2.html
Hope this helps to get into the instance and further debug this issue
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