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Hello.
Generally, this is an error that is output when there is a load on EC2 or more access than the web server can handle.
Are there any issues such as insufficient specifications of the EC2 instance or sudden increase in access?
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All charts are normal. There was a slight jump, but it was 9 hours ago. But web-site is still not working
Can you confirm that the web server is running by using SSH to EC2? I think it's possible that something went wrong and the web server process stopped.