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Based on your scenario, I would suggest verifying a couple of items:
- Verify that the WorkSpace and Ec2 Linux instance are in the same subnet.
- On the security group assigned to the WorkSpace and Ec2 Linux instance, add ensure you've added the target security group the the list. Check this documentation for steps: Rules to connect to instances from an instance with the same security group
- Check to see if you have communication between the two instance by doing ICMP ping or a simple Windows Tracert
If you still encounter problems/error, I would recommend opening an AWS Support Ticket to have a Support Engineer review the logs associated with your account for further troubleshooting. Thanks
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