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TCP port 80:
Your Netstat terminal output shows that the server listens on TCP port 80 only for IPv6 traffic. If you need to use IPv4, enable TCP port 80 for IPv4 on the server too.
TCP port 25:
How did you test TCP port 25? Did the test involve sending outbound (from EC2) traffic to TCP port 25 too?
Amazon Elastic Compute Cloud (Amazon EC2) throttles email traffic over port 25 by default. To avoid timeouts when sending email through the SMTP endpoint from EC2, submit a Request to Remove Email Sending Limitations to remove the throttle. Alternatively, you can send email using a different port, or use an Amazon VPC endpoint.
Documentation reference.
Hello.
Is port 80 allowed in the network ACL of the subnet where EC2 is running?
Additionally, port 25 is originally restricted in AWS.
Has this restriction been lifted?
https://repost.aws/knowledge-center/ec2-port-25-throttle
Port in network ACL is correctly setuped and about port 25 Is usually open I did this procedure 2years ago and everything is good before the restriction from aws team.
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by simply writing on terminal : telnet smtp.xxxxx.com 25 then wait for response usually its work but now its not
If you "telnet" to the instance from a host on the Internet, try the checklist below: