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Hello.
When allowing connections with EC2, security group settings are relevant.
You must ensure that the security group inbound rules configured on EC2 allow the required IP address and port number.
https://docs.aws.amazon.com/AWSEC2/latest/UserGuide/working-with-security-groups.html#describing-security-group
There is also something called a subnet network ACL, but I think this is set to allow all communication if nothing is configured.
https://docs.aws.amazon.com/vpc/latest/userguide/vpc-network-acls.html
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