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You can find step-by-step instructions in the EC2 User Guide under the Troubleshooting topic "I've lost my private key. How can I connect to my Linux instance?". https://docs.aws.amazon.com/AWSEC2/latest/UserGuide/TroubleshootingInstancesConnecting.html#replacing-lost-key-pair
answered a year ago
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Another method is to stop the instance; then mount the instance volume on another instance (you only have to run the second instance temporarily); replace the key file; then restart the first instance.
Here are some instructions - note that this is an external link; the content isn't produced by AWS: https://www.cloudtechtiq.com/blog/how-to-recover-key-file-aws-instance
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If you don't have constraints about turning off the instance, i would suggest that you create an image from the instance, and then create the instance from the image