Sudoers file not reflect changes

Hi, I used detached the old disk, changed sudoers file and added NOPASSWD: Then reactched to the old instance. When I login from putty, it still ask me for newuser password WHICH I know it and got refused ssying not in sudoers that I added it. I added in sudoers before the last @includedir newuser ALL=(ALL) NOPASSWD:ALL I also tried add it in /etc/sudoers.d And NOPASSWD: for other users, when I tried to swtich to root: su root, it says AUTHENTICATION FAILED.

I checked /etc/passwd and shadow files in the old disk, it's correct: newuser:x:1001:1001:old,1,323,323,user:/home/newuser:/bin/bash newuser:$6$/<redacted>

Please help with this AWS EC2 rediculous problem.

Thanks!