Is a Lambda Layer connected to the Cloudshell

I created a lambda layer using cloudshell by installing the requirements to a target folder. Then zipped it and published it as layer, got the arn and applied that layer to a lambda. Now I want to create another layer to add way more dependencies. I tried installing and publishing it as layer, but the Cloudshell gave error "No space left on device". So my question is, if I delete stuff from cloudshell and install new dependencies, will it affect my previously created layer as I and other users are still using that layer. Please guide me in this. Thankyou.