Hi there!

From the notes, I understand that you are not able log on to the WindowsServer2008 R2 on EC2. And the error you getting is “The User Profile Service service failed the log on.”

The “User Profile Service service failed the log on” error could be caused by several reasons, which can be checked on the event viewer application logs by filtering source to User Profile Service, to check the root cause with EC2 rescue. In your case Windows cannot log on because your profile cannot be loaded. This happens because the default profile, stored in C:\Users\Default, is corrupt in some way.

This is how to can solve the issue:

Note: Please make a snapshot before performing below steps, you can check document link [1] for steps on how to create a snapshot.

1. Attach the volume with issue to working instance with the same OS version as secondary drive.
2. Replace X:\Users\Default from the corrupted volume with the C:\Users\Default from the working instance.

I hope you find the above information helpful.

Have a great day ahead!

References:

[1] https://docs.aws.amazon.com/AWSEC2/latest/WindowsGuide/ebs-creating-snapshot.html#ebs-create-snapshot